jacalin biotin  (Vector Laboratories)

Journal: Frontiers in Immunology

Article Title: Antigen Sampling CSF1R -Expressing Epithelial Cells Are the Functional Equivalents of Mammalian M Cells in the Avian Follicle-Associated Epithelium

doi: 10.3389/fimmu.2019.02495

Figure Lengend Snippet: Lectins used in this study.

Article Snippet: Jacalin—biotin (B-1155) , O-glycoproteins , 1:500 , Vector Laboratories, UK.

Techniques: Plasmid Preparation

Journal: Science immunology

Article Title: Enzymatic Synthesis of Core 2 O-Glycans Governs the Tissue-Trafficking Potential of Memory CD8 + T Cells

doi: 10.1126/sciimmunol.aan6049

Figure Lengend Snippet: (A) Expression of CD62L and KLRG1 on primary and tertiary memory P14 CD8+ T cells 90 days post-LCMV infection and (B) binding to E- and P-selectin. (C,D) Quantification of (B). (E) Expression of CD122 and CD132. (F,G) Quantification of (E). (H) Expression of total core 1 O-glycans (Jacalin) and unsialylated core 1 O-glycans (PNA). (I,J) Quantification of (H). (K) Primary and tertiary memory P14 CD8+ T cells were purified and stimulated with the indicated concentration of IL-15 for 15 minutes and phosphorylation of STAT5 (Y694) was analyzed by immunoblot. (L) Primary and tertiary memory CD8+ T cells were purified and cultured with IL-15 for 3 days. Binding of MAL II, PNA and the 1B11 antibody was analyzed. (M) Same as (L) except the glycosylation of PSGL-1 was analyzed by immunoblot. (N) Same as (L) except binding to E-selectin was analyzed by flow cytometry. (O) Quantification of (N) from 4 independent experiments. (P,Q) Same as (N,O) except for P-selectin.

Article Snippet: Lectin and Selectin Binding Fluorescein or biotin-conjugated Jacalin, Peanut Agglutinin (PNA), and MAL II (VectorLabs) were incubated with cells for 30 minutes in 1% FBS/PBS at room temperature.

Techniques: Expressing, Infection, Binding Assay, Purification, Concentration Assay, Western Blot, Cell Culture, Flow Cytometry